ELISA/Western blot tests for HIV
Human immunodeficiency virus (HIV) is an infectious disease that attacks the immune system. If it is not treated, it can develop into acquired ...

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About HIV

Human immunodeficiency virus (HIV) is an infectious disease that attacks the immune system. If it is not treated, it can develop into acquired immunodeficiency syndrome (AIDS), which is a prolonged and fatal illness. HIV is spread through vaginal, oral, or anal sexual contact. It is also be spread through blood, blood factor products, injection drug use, and breast milk.

What Is the ELISA/Western Blot Test?

A series of blood screenings are performed to test for HIV. The enzyme-linked immuno assay (ELISA or EIA) is the first test that your doctor will order to screen for HIV. ELISA and the Western blot test detect HIV antibodies in your blood. Antibodies are proteins your immune system produces in response to the presence of foreign substances such as viruses. If you test positive for HIV on the ELISA test, your provider will order the Western blot test to confirm HIV infection.

When Is this Test Recommended?

The ELISA and Western blot tests are recommended if you have been exposed to HIV or are at risk for contracting HIV. Those at risk for HIV include:

  • intravenous drug users
  • people who have unprotected sex, especially with someone who has HIV or has an unknown HIV status
  • people who have had sexually transmitted diseases (STDs)
  • people who had blood transfusions or blood clotting factor injections before 1985

You may opt to have the test done if you are uncertain about your HIV status, even if you are not in a high-risk group. It is a good idea to be tested on a regular basis if you participate in high-risk behaviors, such as intravenous drug use or unprotected sex.

How Do I Prepare for the Test?

You do not need to do anything to prepare for the ELISA or Western blot test. It takes very little time to give a blood sample, but it may take several days or weeks to get the results. Make sure to tell your doctor if you have a fear of needles or faint at the site of blood.

What Happens During the Test?

Before having these tests, you will probably need to sign a consent form. The test and procedure should be explained to you. The procedure for getting a sample of your blood is the same for both tests. A medical professional will:

  • clean your inner arm with a solution to remove any bacteria or germs that can cause infection
  • apply a tourniquet, or elastic band around your arm to allow the vein to fill with blood in your veins
  • place a needle into one of your veins and draw a small sample of blood into a tube
  • remove the needle and apply a bandage

You will be asked to elevate or flex your arm to reduce the blood flow after the test.

Giving a blood sample is not painful, though you may feel a sting or a pricking sensation as the needle goes into your vein. Your arm may throb slightly after the procedure.

The blood sample will be sent to a laboratory for analysis. For the ELISA test, a lab technician adds the sample to a petri dish containing HIV antigen. An antigen is any foreign substance, such as a virus, that causes your immune system to respond.

If your blood contains antibodies to HIV, it will bind with the antigen. The technician will check this by adding an enzyme to the petri dish and watching how your blood and the antigen react. If the contents of the dish change color, you may have HIV.

The general process of a Western blot test is similar. However, the Western blot method is more complicated. It involves separating the HIV sample into its component proteins using an electrical current. Then, these proteins are transferred to a special kind of paper (blotting) and reacted with your blood sample. An enzyme is used to cause color change and detect antibodies.

Are There Any Risks?

These tests are very safe, but rare complications can occur. For example, you may:

  • feel lightheaded or faint, especially if you have a fear of needles or blood
  • get an infection at the site of the venipuncture
  • develop a bruise at the puncture site
  • have trouble stopping the bleeding

Make sure to tell your doctor if you’ve had trouble giving blood in the past, bruise easily, or suffer from a bleeding disorder such as hemophilia. Contact your healthcare provider right away if you experience any of these complications.

What Do the Test Results Mean?

If you test positive for HIV on the ELISA test, you might have HIV. However, sometimes there are false positives with the ELISA screen. This means that test results indicate that you have HIV when you actually do not. Further testing is required to confirm whether you have the disease. For example, having Lyme disease, syphilis, or lupus may produce a false positive for HIV in an ELISA test.

If you test positive on the ELISA screen, your doctor will order a Western blot test. If you test positive for HIV with the Western blot test, you probably have HIV.

Sometimes, HIV does not show up on the ELISA test even though you are infected. This can happen if someone is in the early stages of the infection, and his or her body has not produced enough antibodies to the virus for the tests to detect. This early stage of the disease, in which a person has HIV but tests negative for it, is known as the “window period.”

According to the Centers for Disease Control and Prevention (CDC), a person’s window period is usually between two and eight weeks, and the average is 25 days. However, in rare cases, some people can take as long as six months to develop antibodies (CDC, 2010).

After the Test

Though both tests are simple and straightforward, waiting for the results can create anxiety, and a positive test result can be emotionally devastating. Your doctor can refer you to counseling or HIV support groups.

Although HIV is very serious, there is medication that can help prevent the disease from developing into AIDS, so that you can live a full and long life. The earlier you discover your HIV status, the earlier you can begin treatment to prevent health complications or transmission of the infection to others.

Written by: Tricia Kinman
Edited by:
Medically Reviewed by: Brenda B. Spriggs, MD, MPH, FACP
Published: Jul 19, 2012
Published By: Healthline Networks, Inc.
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