Is a Factor VIII Assay Test?
Your doctor may recommend a factor VIII assay test to determine whether
your body produces an appropriate level of this particular coagulation factor.
Your body needs factor VIII in order to form blood clots.
Each time you bleed, it triggers a series of reactions known as
the “coagulation cascade.” Coagulation is the process your body uses to stop
blood loss. Cells called platelets create a plug to cover the damaged tissue,
and then your body’s 13 clotting factors interact to produce a blood clot. Low
levels of any of these clotting factors can prevent a clot from forming.
the Test Addresses
This test is used to determine the cause of prolonged or
excessive bleeding. Your doctor may recommend the test if you have a family
history of bleeding disorders or if you have experienced any of the following symptoms:
- abnormal or excessive bleeding
- easy bruising
- heavy or prolonged menstrual periods
- frequent gum bleeding
- frequent nosebleeds
This test may also be ordered if your doctor believes you have an
acquired condition that’s causing your bleeding disorder, such as:
- vitamin K deficiency
- liver disease
This assay can also determine whether you’re suffering from an
inherited factor VIII deficiency, particularly if you’ve been experiencing
bleeding episodes since you were a child or if a member of your family has an
inherited factor deficiency. In the latter case, your close relatives may also
be tested to help confirm your diagnosis.
An inherited factor VIII deficiency is called hemophilia A. This
hereditary condition only affects males because it’s linked to a defective gene
on the X chromosome. Females have two X chromosomes, so if one has a defective
gene, the other can still create enough factor VIII.
If you’ve already been diagnosed with a factor VIII deficiency
and are undergoing treatment, your doctor may order this test to determine the
effectiveness of your treatment.
for the Test
No special preparation is necessary for this test. You should
tell your doctor if you’re taking any blood-thinning medications, such as warfarin
(Coumadin) or aspirin.
Your doctor may advise you to stop taking blood thinners before the test.
the Test Is Administered
To perform the test, your doctor will need to take a sample of
blood from your arm. First, the site will be cleaned with an alcohol swab. Then
your doctor will insert a needle into your vein and attach a tube to the needle
to collect blood. When enough blood has been collected, the needle will be
removed and the site covered with a gauze pad. The blood sample will be sent to
a laboratory for analysis.
A normal result for a factor VIII assay should be around 100
percent of the laboratory reference value. Your doctor will explain the
specifics of your results.
If you have an abnormally low level of factor VIII, it could be
If you have an abnormally high level of factor VIII, it may be
Are the Risks of the Test?
As with any blood test, there’s a slight risk of bruising or
bleeding at the puncture site. In rare cases, the vein may become swollen after
blood is drawn. Such a condition is known as phlebitis and can be treated by
applying a warm compress several times a day.
Ongoing bleeding could be a problem if you suffer from a bleeding
disorder or are taking blood-thinning medication, such as warfarin or aspirin.
to Expect After the Test
If you’ve been diagnosed with a factor VIII deficiency, your
doctor will prescribe replacement concentrates of factor VIII. The amount you
require will depend on:
- your age
- your height and weight
- the severity of your bleeding
- the site of your bleeding
To help prevent a bleeding emergency, your doctor will teach you
and your family how to administer factor VIII concentrates at home at the first
sign of bleeding.
If your levels of factor VIII are too high, you are at a higher
risk for thrombosis
(an obstructive blood clot in your vein). In this case, your doctor may perform
additional tests or prescribe anticoagulants.