Bilirubin is a brownish-yellow pigment that’s produced when
red blood cells break down. The substance is found in the liver and is normally
passed out of the body during digestion. The natural process of red blood cells
dying while new cells are produced means that everyone has bilirubin in their
Bilirubin only becomes dangerous when it accumulates in the
bloodstream. High levels of bilirubin can cause jaundice, which is a medical condition that causes your skin to look yellow.
This can be a sign of liver disease in children and adults.
Bilirubin levels are affected by age and overall health. For
babies, bilirubin that measures higher than 20-25 milligrams per deciliter can
lead to neurological damage. This is why it’s important to check their
bilirubin levels. A urine test is one way of measuring how much bilirubin you
have in your body.
Preparing for Bilirubin Testing
Some prescription drugs can cause a false positive, or a
higher-than-normal reading of bilirubin in your body. False positives indicate
high bilirubin levels that aren’t necessarily associated with liver disease.
Discuss your medication usage with your doctor.
If you routinely take the following drugs, you may be asked
to stop taking them for a couple of days before your bilirubin testing:
Visit your local medical testing lab to get the special
container needed to collect your urine. This is usually a large plastic jug
fitted with a screw-top cover.
Bilirubin Testing Procedure
Testing bilirubin levels through urinalysis is
painless, but it requires a time commitment and attention to detail. A thorough
and accurate test requires you to collect your urine over the course of 24
On the day you begin the bilirubin urine test, don’t collect
urine the first time you urinate. This is because your first specimen of urine
is usually more concentrated. After the first time you urinate, start
collecting your urine. This means each time you urinate throughout the day,
collect the urine in the container rather than flushing it down the toilet.
You may use a clean, small cup to collect the urine and pour
it into the container to make the process easier. Cover the container tightly
after each use and store it in the refrigerator during the 24-hour collection
Collect your urine again on the morning of the second day.
This is your last collection, and it should be your first urination of the
morning. Label the container with your name and the date, and then return the
full container to the lab facility.
Bilirubin Testing in Infants
Newborn babies often have jaundice. This is because infants’
livers aren’t as efficient at removing bilirubin from the body. Increased
bilirubin and the resulting jaundice can be dangerous to infants if the
underlying condition isn’t treated.
Testing bilirubin through urine may be challenging for
parents of infants being tested. The lab will supply you with urine collection
bags to fit into your child’s diaper. Place the bag over your baby’s penis or
labia and put on their diaper as usual.
Use a fresh bag at each diaper change. Put the contents of
each collection bag into the collection container.
Results of Bilirubin Testing
The results of a bilirubin 24-hour urine test should be
available within a few days after you’ve completed the test. Your doctor will
notify you about the results.
In healthy people, bilirubin isn’t present in urine. If your
test shows bilirubin present, you may need to undergo blood tests to measure
your bilirubin levels and liver function.